Confocal Microscopy Laboratory

A Confocal Microscope

A confocal microscope is an optical light microscope that uses lasers of varying wavelengths to excite fluorescent dyes which have been specifically added to samples, or samples that are inherently fluorescent. The confocal microscope is useful for determining the presence and location of specific structures, cellular organelles, and proteins on or within samples.  Antibodies to proteins of interest can be labeled with fluorescent compounds and then used to identify the location and amount of these proteins present within samples. 

Staff:

  • Marek Michalski, MSc

 

Equipment:

  • Zeiss Elyra LSM 980 super-resolution imaging system, including confocal and SIM² super-resolution techniques (Structured Illumination Microscopy) integrated on an inverted microscope stand. Microscope is equipped with Airyscan 2 area detector and is combined with Elyra 7,
  • Microfluidics – Incubation and Perfusion System for living cells.

Laboratory services

Laboratory services allow gentle live cell super-resolution imaging down to 60 nm resolution with high speed up to 255 fps in 6 different imaging modes, including lossless super-resolution imaging. In addition to a confocal microscope, an incubation and perfusion system allows studies of living cells.